The DOT provides the regulatory framework that employers who operate under a particular DOT agency must adhere to. These regulations cover a wide range of companies and employees in safety-sensitive industries to ensure they’re operating safely and responsibly.

Who is Subject to DOT Drug Testing?

  • Federal Motor Carrier Safety Administration (FMCSA) – Drivers of Commercial Motor Vehicles (CMV) or those who hold a Commercial Driver’s License (CDL), vehicles required to have a DOT placard or those who transport hazardous materials.
  • Pipeline & Hazardous Materials Safety Administration (PHMSA) – operations, emergency, and maintenance staff members.
  • Federal Railroad Administration (FRA and FRAMOW) – hours of service employees, roadway workers, “regulated service” contractors, train and engine service, dispatching, maintenance of way, and signal employees.
  • Federal Transit Administration (FTA) – armed security personnel, mechanics, controllers, and drivers/operators of mass transit vehicles.
  • Federal Aviation Administration (FAA) – flight crew (including attendants), ground security controllers, and aircraft dispatchers.
  • Department of Homeland Security; including U.S. Coast Guard (USCG) – crew members and those operating a commercial vessel.